4E0S
Crystal Structure of C5b-6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 298 |
| Detector technology | PIXEL |
| Collection date | 2012-01-07 |
| Detector | PSI PILATUS 6M |
| Spacegroup name | I 21 21 21 |
| Unit cell lengths | 158.949, 227.529, 278.157 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.930 - 4.210 |
| R-factor | 0.221 |
| Rwork | 0.218 |
| R-free | 0.27800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 3IDH 3prx 3t5o |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.649 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 4.400 |
| High resolution limit [Å] | 4.200 | 4.200 |
| Rmerge | 0.160 | 0.560 |
| Number of reflections | 28605 | |
| <I/σ(I)> | 5.7 | 1.6 |
| Completeness [%] | 82.0 | 67 |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.6 | 298 | 0.08M lithium chloride, 0.01M imidazole-HCl, pH 7.6, SMALL TUBES, temperature 298K |
