4E0S
Crystal Structure of C5b-6
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 298 |
Detector technology | PIXEL |
Collection date | 2012-01-07 |
Detector | PSI PILATUS 6M |
Spacegroup name | I 21 21 21 |
Unit cell lengths | 158.949, 227.529, 278.157 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.930 - 4.210 |
R-factor | 0.221 |
Rwork | 0.218 |
R-free | 0.27800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB ENTRIES 3IDH 3prx 3t5o |
RMSD bond length | 0.010 |
RMSD bond angle | 1.649 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 4.400 |
High resolution limit [Å] | 4.200 | 4.200 |
Rmerge | 0.160 | 0.560 |
Number of reflections | 28605 | |
<I/σ(I)> | 5.7 | 1.6 |
Completeness [%] | 82.0 | 67 |
Redundancy | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.6 | 298 | 0.08M lithium chloride, 0.01M imidazole-HCl, pH 7.6, SMALL TUBES, temperature 298K |