4DYO

Crystal Structure of Human Aspartyl Aminopeptidase (DNPEP) in complex with Aspartic acid Hydroxamate

Replaces:  3L6S

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	CCD
Collection date	2009-11-25
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9763
Spacegroup name	F 4 3 2
Unit cell lengths	244.595, 244.595, 244.595
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	54.690 - 2.200
R-factor	0.156
Rwork	0.155
R-free	0.19470
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ijz
RMSD bond length	0.015
RMSD bond angle	1.522
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.5.0089)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	56.110	2.320
High resolution limit [Å]	2.200	2.200
Rmerge	0.179	0.833
Number of reflections	32185
<I/σ(I)>	10.4	2.2
Completeness [%]	99.6	97.5
Redundancy	10.8	8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293.15	15% PEG3350, 0.25 M magnesium chloride, 0.1 M Tris, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293.15K