4DVI
Crystal structure of Tankyrase 1 with IWR2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-11-18 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97740 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 41.470, 77.940, 146.540 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.900 |
| Rwork | 0.248 |
| R-free | 0.25800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.105 | 0.790 |
| Number of reflections | 37889 | |
| Completeness [%] | 99.9 | 99.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277 | 100 mM MES pH 6.0, 0.2 or 0.4 M Di-Ammonium Tartrate, 12.5-25% PEG3350, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
