4DRB

The crystal structure of FANCM bound MHF complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2011-01-19
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.9792
Spacegroup name	P 1 21 1
Unit cell lengths	111.317, 70.025, 115.751
Unit cell angles	90.00, 91.41, 90.00

Refinement procedure

Resolution	49.670 - 2.634
R-factor	0.2223
Rwork	0.221
R-free	0.25560
Structure solution method	SAD
RMSD bond length	0.002
RMSD bond angle	0.539
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	AutoSol
Refinement software	PHENIX ((phenix.refine: 1.6_289))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.730
High resolution limit [Å]	2.634	2.634
Rmerge		0.459
Number of reflections	52925
<I/σ(I)>		4.62
Completeness [%]	100.0	100
Redundancy		6.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	285	0.1M Tris-HCl, pH 8.5, 0.2 M (NH4)2SO4, 25% PEG 3350 , VAPOR DIFFUSION, HANGING DROP, temperature 285K