4DJE

Crystal structure of folate-bound corrinoid iron-sulfur protein (CFeSP) in complex with its methyltransferase (MeTr), co-crystallized with folate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2011-08-15
Detector	ADSC QUANTUM 315
Wavelength(s)	1.604
Spacegroup name	P 21 21 2
Unit cell lengths	136.419, 250.664, 82.427
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.463 - 3.504
R-factor	0.243
Rwork	0.240
R-free	0.28950
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.785
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7.2_869))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.630
High resolution limit [Å]	3.500	3.500
Number of reflections	34890
Completeness [%]	96.3	90.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	100 mM Bis-Tris, 100 mM calcium acetate, 9% PEG 5000 MME, 20% glycerol, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K