4DGL
Crystal Structure of the CK2 Tetrameric Holoenzyme
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Detector technology | CCD |
| Collection date | 2011-04-09 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.873 |
| Spacegroup name | P 63 |
| Unit cell lengths | 175.652, 175.652, 96.260 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 152.119 - 3.000 |
| R-factor | 0.1966 |
| Rwork | 0.195 |
| R-free | 0.22170 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.597 |
| Data scaling software | SCALA (3.3.20) |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 152.119 | 44.863 | 3.160 |
| High resolution limit [Å] | 3.000 | 9.490 | 3.000 |
| Rmerge | 0.027 | 0.990 | |
| Total number of observations | 20037 | 94661 | |
| Number of reflections | 34036 | ||
| <I/σ(I)> | 23.9 | 94.6 | 3.1 |
| Completeness [%] | 100.0 | 99.1 | 100 |
| Redundancy | 19.1 | 17.8 | 19.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 293 | PEG 3350 18%, 0.2 M sodium malonate, pH 7, vapor diffusion, temperature 293K |
