4DDG
Crystal structure of human OTUB1/UbcH5b~Ub/Ub
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 198.25 |
Detector technology | CCD |
Collection date | 2011-12-12 |
Detector | RAYONIX MX-300 |
Wavelength(s) | 0.97949 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 134.566, 104.928, 148.479 |
Unit cell angles | 90.00, 104.19, 90.00 |
Refinement procedure
Resolution | 49.121 - 3.299 |
R-factor | 0.2356 |
Rwork | 0.233 |
R-free | 0.28020 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2ESK AND 2ZFY |
RMSD bond length | 0.004 |
RMSD bond angle | 0.938 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.290 | 3.500 |
High resolution limit [Å] | 3.299 | 3.299 |
Number of reflections | 60387 | |
Completeness [%] | 99.5 | 97.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 25% PEG1500, 0.1 M SPG, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |