4D90

Crystal Structure of Del-1 EGF domains

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2010-03-07
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9794
Spacegroup name	P 32
Unit cell lengths	82.680, 82.680, 56.422
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.317 - 2.601
R-factor	0.207
Rwork	0.204
R-free	0.25890
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2vj3
RMSD bond length	0.010
RMSD bond angle	1.460
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.7.3_928)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.660
High resolution limit [Å]	2.600	6.410	2.600
Rmerge	0.072	0.046	0.777
Number of reflections	13233
<I/σ(I)>	18
Completeness [%]	100.0	99.8	100
Redundancy	5.6	5.6	5.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	298	100 mM sodium acetate, 30% PEG 4000, 200 mM ammonium acetate, pH 4.6, vapor diffusion, hanging drop, temperature 298K
1	VAPOR DIFFUSION, HANGING DROP	4.6	298	100 mM sodium acetate, 30% PEG 4000, 200 mM ammonium acetate, pH 4.6, vapor diffusion, hanging drop, temperature 298K