4D8K
Crystal structure of a SH3-SH2 domains of a lymphocyte-specific protein tyrosine kinase (LCK) from Homo sapiens at 2.36 A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-10-16 |
Detector | ADSC QUANTUM 315 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 68.899, 68.899, 80.283 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.834 - 2.360 |
R-factor | 0.194 |
Rwork | 0.191 |
R-free | 0.25130 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.176 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.3.20) |
Phasing software | PHASER (2.3.0) |
Refinement software | PHENIX (1.7.2) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 29.834 | 29.834 | 2.420 |
High resolution limit [Å] | 2.360 | 10.550 | 2.360 |
Rmerge | 0.024 | 0.865 | |
Total number of observations | 527 | 3254 | |
Number of reflections | 9434 | ||
<I/σ(I)> | 9.3 | 23.9 | 0.9 |
Completeness [%] | 99.9 | 95.5 | 100 |
Redundancy | 4.9 | 4.1 | 4.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 1.5M lithium sulfate, 0.1M HEPES pH 7.5, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K |