4D3C
Crystal structure of the NK1 domain of HGF in complex with anti-HGF monoclonal antibody SFN68.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS |
| Synchrotron site | PAL/PLS |
| Temperature [K] | 93.15 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 86.703, 297.692, 70.713 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.790 - 2.620 |
| R-factor | 0.26913 |
| Rwork | 0.268 |
| R-free | 0.29758 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 1JPT 1NK1. |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.456 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 29.790 |
| High resolution limit [Å] | 2.620 |
| Rmerge | 0.070 |
| Number of reflections | 25541 |
| Completeness [%] | 95.5 |
| Redundancy | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
