4D2N
Crystal structure of deglycosylated serum-derived human IgG4 Fc
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-02-23 |
| Detector | DECTRIS PILATUS 2M |
| Spacegroup name | P 6 2 2 |
| Unit cell lengths | 196.945, 196.945, 96.956 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 53.682 - 2.700 |
| R-factor | 0.207 |
| Rwork | 0.204 |
| R-free | 0.25440 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4c54 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.603 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.700 | 2.830 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.180 | |
| Number of reflections | 30902 | |
| <I/σ(I)> | 16.9 | 1.6 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 20 | 20 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 291 | CRYSTALS WERE GROWN AT 291K USING A RESERVOIR OF 100MM BIS-TRIS PROPANE PH7.5, 20% PEG 3350 AND 200MM SODIUM CITRATE, AND A PROTEIN CONCENTRATION OF 6MG/ML. |
