4D2H
Crystal structure of the tetramerisation domain of human CtIP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-06-17 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 35.970, 37.910, 97.790 |
| Unit cell angles | 90.00, 90.02, 90.00 |
Refinement procedure
| Resolution | 20.160 - 1.900 |
| R-factor | 0.2144 |
| Rwork | 0.212 |
| R-free | 0.25140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.880 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.980 | 1.940 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.060 | 0.920 |
| Number of reflections | 20994 | |
| <I/σ(I)> | 10.2 | 1.6 |
| Completeness [%] | 99.5 | 99.5 |
| Redundancy | 3.6 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 200 MM LITHIUM SULPHATE, 100 MM SODIUM ACETATE PH 3.6, 32% (V/V) PEG 400 |
