4D2H
Crystal structure of the tetramerisation domain of human CtIP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-06-17 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 35.970, 37.910, 97.790 |
Unit cell angles | 90.00, 90.02, 90.00 |
Refinement procedure
Resolution | 20.160 - 1.900 |
R-factor | 0.2144 |
Rwork | 0.212 |
R-free | 0.25140 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 0.880 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.980 | 1.940 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.060 | 0.920 |
Number of reflections | 20994 | |
<I/σ(I)> | 10.2 | 1.6 |
Completeness [%] | 99.5 | 99.5 |
Redundancy | 3.6 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 200 MM LITHIUM SULPHATE, 100 MM SODIUM ACETATE PH 3.6, 32% (V/V) PEG 400 |