4CXA
Crystal structure of the human CDK12-cyclin K complex bound to AMPPNP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-02-07 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 69.843, 138.791, 71.888 |
| Unit cell angles | 90.00, 104.99, 90.00 |
Refinement procedure
| Resolution | 30.529 - 3.150 |
| R-factor | 0.2239 |
| Rwork | 0.221 |
| R-free | 0.27910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4CJY |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.868 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.530 | 3.370 |
| High resolution limit [Å] | 3.150 | 3.150 |
| Rmerge | 0.190 | 0.980 |
| Number of reflections | 22908 | |
| <I/σ(I)> | 6.4 | 1.5 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 5 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 0.15M DL-MALIC ACID, 20% PEG3350, pH 7 |
