4CIN
Complex of Bcl-xL with its BH3 domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 73.257, 73.257, 136.864 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.525 - 2.693 |
| R-factor | 0.1967 |
| Rwork | 0.193 |
| R-free | 0.27080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2p1l |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.149 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 46.520 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.090 | 0.940 |
| Number of reflections | 12063 | |
| <I/σ(I)> | 10.9 | 1.03 |
| Completeness [%] | 98.0 | 97.7 |
| Redundancy | 2.9 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 40% (V/V) PEG400, 0.2M CALCIUM ACETATE, 0.1M HEPES, PH7.5 |
