4CH2
Low-salt crystal structure of a thrombin-GpIbalpha peptide complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-04-29 |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 149.570, 50.560, 76.280 |
| Unit cell angles | 90.00, 97.15, 90.00 |
Refinement procedure
| Resolution | 37.100 - 1.600 |
| R-factor | 0.15616 |
| Rwork | 0.155 |
| R-free | 0.18730 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ppb |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.067 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.200 | 1.690 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.080 | 0.500 |
| Number of reflections | 72363 | |
| <I/σ(I)> | 9.6 | 1.8 |
| Completeness [%] | 96.8 | 98.4 |
| Redundancy | 2.5 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.4 | 50 MM SODIUM FORMATE, 16% PEG-3350, pH 7.4 |
