4CG4
Crystal structure of the CHS-B30.2 domains of TRIM20
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-09-13 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 69.820, 388.210, 70.880 |
| Unit cell angles | 90.00, 116.52, 90.00 |
Refinement procedure
| Resolution | 48.526 - 2.400 |
| R-factor | 0.214 |
| Rwork | 0.211 |
| R-free | 0.26070 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wl1 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.378 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.530 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.170 | |
| Number of reflections | 129351 | |
| <I/σ(I)> | 9.77 | 0.88 |
| Completeness [%] | 98.9 | 93.1 |
| Redundancy | 7.4 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.1 M TRIS-ACOH PH 7.4-8, 0.26% (W/V) CYSTAMINE-HCL, 0.2 M LISO4, 8% (W/V) PEG 20000, 8% (W/V) PEG 550 MME |
