4C8D
Crystal structure of JmjC domain of human histone 3 Lysine-specific demethylase 3B (KDM3B)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-09-27 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 83.340, 85.610, 117.130 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 69.120 - 2.180 |
| R-factor | 0.19651 |
| Rwork | 0.194 |
| R-free | 0.23911 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ypd |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.165 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 69.120 | 2.250 |
| High resolution limit [Å] | 2.180 | 2.180 |
| Rmerge | 0.050 | 0.380 |
| Number of reflections | 22231 | |
| <I/σ(I)> | 12.2 | 2.8 |
| Completeness [%] | 99.9 | 99.7 |
| Redundancy | 4.3 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1M BIS-TRIS PH 5.5, 0.1M AMMONIUM ACETATE, 27% PEG 3350 |
