4C7A
Crystal structure of the Smoothened CRD, selenomethionine-labeled
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 68.130, 68.130, 92.270 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.470 - 2.300 |
| R-factor | 0.23803 |
| Rwork | 0.236 |
| R-free | 0.28406 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.604 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.000 | 2.360 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.100 | 0.920 |
| Number of reflections | 10085 | |
| <I/σ(I)> | 25.5 | 3.9 |
| Completeness [%] | 99.2 | 92.8 |
| Redundancy | 26.5 | 23.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 100 MM HEPES PH7.0, PEG 6000 20%, 10 MM ZNCL2 |
