4C68
Plasmodium vivax N-myristoyltransferase in complex with a peptidomimetic inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-01-22 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 57.330, 121.430, 178.740 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.970 - 1.380 |
| R-factor | 0.22189 |
| Rwork | 0.220 |
| R-free | 0.25989 |
| Structure solution method | OTHER |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.025 |
| RMSD bond angle | 2.490 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.000 | 1.450 |
| High resolution limit [Å] | 1.380 | 1.380 |
| Rmerge | 0.110 | 0.580 |
| Number of reflections | 256405 | |
| <I/σ(I)> | 13.4 | 2.8 |
| Completeness [%] | 99.8 | 99 |
| Redundancy | 8.1 | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | 0.2 M AS, 25% PEG 3350, 0.1 M BIS-TRIS PH 5.5 |
