4C55

Crystal structure of serum-derived human IgG4 Fc

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Collection date	2010-07-10
Spacegroup name	P 21 21 21
Unit cell lengths	74.298, 80.738, 99.190
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.595 - 2.350
R-factor	0.1903
Rwork	0.188
R-free	0.23810
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4c54
RMSD bond length	0.009
RMSD bond angle	1.123
Data reduction software	XDS (IN XIA2 PACKAGE)
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX ((PHENIX.REFINE: 1.8.2_1309))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	80.740	2.450
High resolution limit [Å]	2.350	2.350
Rmerge	0.100
Number of reflections	25471
<I/σ(I)>	14.4	1.6
Completeness [%]	99.7	99.9
Redundancy	10.7	11.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.5		MES PH6.5. 18-20% PEG 20 000