4C55
Crystal structure of serum-derived human IgG4 Fc
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Collection date | 2010-07-10 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 74.298, 80.738, 99.190 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.595 - 2.350 |
R-factor | 0.1903 |
Rwork | 0.188 |
R-free | 0.23810 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4c54 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.123 |
Data reduction software | XDS (IN XIA2 PACKAGE) |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | PHENIX ((PHENIX.REFINE: 1.8.2_1309)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 80.740 | 2.450 |
High resolution limit [Å] | 2.350 | 2.350 |
Rmerge | 0.100 | |
Number of reflections | 25471 | |
<I/σ(I)> | 14.4 | 1.6 |
Completeness [%] | 99.7 | 99.9 |
Redundancy | 10.7 | 11.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.5 | MES PH6.5. 18-20% PEG 20 000 |