4C2S
Crystal structure of the fucosylgalactoside alpha N- acetylgalactosaminyltransferase (GTA P156L mutant) in complex with UDP and deoxy-H-antigen acceptor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-2 |
| Synchrotron site | MAX II |
| Beamline | I911-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-05-10 |
| Detector | MARRESEARCH 165 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 147.510, 52.610, 80.270 |
| Unit cell angles | 90.00, 89.97, 90.00 |
Refinement procedure
| Resolution | 36.880 - 2.480 |
| R-factor | 0.14693 |
| Rwork | 0.145 |
| R-free | 0.18203 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2y7a |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.376 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.500 | 2.590 |
| High resolution limit [Å] | 2.460 | 2.460 |
| Rmerge | 0.130 | 0.320 |
| Number of reflections | 21693 | |
| <I/σ(I)> | 11.6 | 3.2 |
| Completeness [%] | 96.8 | 78 |
| Redundancy | 3.6 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 5 MM MOPS PH 7.0, 1 MM MNCL2, 1 MM DTT, 0.1 M AMSO4, 10% (W/V) PEG 3350 |
