4C04
Crystal structure of M. musculus protein arginine methyltransferase PRMT6 with inhibitor
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2011-11-09 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | I 41 |
Unit cell lengths | 78.835, 78.835, 118.472 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.792 - 1.576 |
R-factor | 0.1527 |
Rwork | 0.151 |
R-free | 0.18530 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2y1w |
RMSD bond length | 0.010 |
RMSD bond angle | 1.321 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.790 | 1.630 |
High resolution limit [Å] | 1.580 | 1.580 |
Rmerge | 0.050 | 0.870 |
Number of reflections | 49529 | |
<I/σ(I)> | 14.84 | 1.77 |
Completeness [%] | 99.6 | 96.6 |
Redundancy | 5.2 | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | 100 MM HEPES PH 7.0, 200 MM MGCL2, 20% PEG 3350 |