4BZC
Crystal structure of the tetrameric dGTP-bound wild type SAMHD1 catalytic core
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 79.002, 138.727, 93.715 |
| Unit cell angles | 90.00, 113.56, 90.00 |
Refinement procedure
| Resolution | 49.710 - 2.880 |
| R-factor | 0.19307 |
| Rwork | 0.190 |
| R-free | 0.24480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3u1n |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.673 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.930 |
| High resolution limit [Å] | 2.880 | 2.880 |
| Rmerge | 0.120 | 0.900 |
| Number of reflections | 40369 | |
| <I/σ(I)> | 8.2 | 1.2 |
| Completeness [%] | 95.6 | 97.4 |
| Redundancy | 2.9 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.7 | 100 MM BIS-TRIS PH 6.7 AND 25% (W/V) PEG1500 |
