4BW5
Crystal structure of human two pore domain potassium ion channel TREK2 (K2P10.1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-12-13 |
| Detector | DECTRIS PILATUS |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 87.830, 96.960, 103.700 |
| Unit cell angles | 90.00, 92.58, 90.00 |
Refinement procedure
| Resolution | 39.570 - 3.200 |
| R-factor | 0.2377 |
| Rwork | 0.237 |
| R-free | 0.25400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 3UM7 3ukm |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.930 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.570 | 3.280 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.050 | 1.140 |
| Number of reflections | 28707 | |
| <I/σ(I)> | 10.7 | 1.1 |
| Completeness [%] | 99.4 | 99 |
| Redundancy | 3.8 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 277 | 0.001M CADMIUM CHLORIDE, 0.2M POTASSIUM CHLORIDE,31%(V/V) PEG400,0.1M HEPES PH 8.0, 2% (W/V) BENZAMIDINE, TEMPERATURE 277K |
