4BTF
Structure of MLKL
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-04-08 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.953696, 1.5498 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 42.400, 78.300, 162.938 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.033 - 2.604 |
| R-factor | 0.2093 |
| Rwork | 0.207 |
| R-free | 0.25550 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.104 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | autoSHARP |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.150 | 0.690 |
| Number of reflections | 16375 | |
| <I/σ(I)> | 11.13 | 2.05 |
| Completeness [%] | 94.3 | 82.2 |
| Redundancy | 6.5 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.4M SODIUM FORMATE, 25% W/V PEG2000 MONOMETHYL ETHER, 50MM TRIS PH 7.5, 5MM TCEP |
