4BSW
Heterodimeric Fc Antibody Azymetric Variant 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08B1-1 |
| Synchrotron site | CLSI |
| Beamline | 08B1-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-09-12 |
| Detector | MARRESEARCH MX300HE |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 49.677, 74.715, 148.870 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.260 - 2.150 |
| R-factor | 0.20296 |
| Rwork | 0.200 |
| R-free | 0.25937 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2j6e |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.526 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.000 | 2.210 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.070 | 0.500 |
| Number of reflections | 33200 | |
| <I/σ(I)> | 15.9 | 4 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 6.8 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | VIA HANGING DROP VAPOR DIFFUSION METHOD AT A RATIO OF 2:1 OF 8.5 MG/ML OF AZYMETRIC VARIANT 2 ABOVE A MOTHER LIQUOR SOLUTION COMPOSED OF 5% (V/V) ETHYLENE GLYCOL, 18% (W/V) POLYETHYLENE GLYCOL 3350, AND 0.15 M AMMONIUM IODIDE WITH AID OF MICROSEEDING. |
