4BSV

Heterodimeric Fc Antibody Azymetric Variant 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08B1-1
Synchrotron site	CLSI
Beamline	08B1-1
Temperature [K]	100
Detector technology	CCD
Collection date	2012-09-12
Detector	MARRESEARCH MX-300HE
Spacegroup name	P 21 21 21
Unit cell lengths	49.540, 74.920, 148.920
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.380 - 1.750
R-factor	0.17926
Rwork	0.178
R-free	0.20821
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2j6e
RMSD bond length	0.008
RMSD bond angle	1.317
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.000	1.840
High resolution limit [Å]	1.750	1.750
Rmerge	0.040	0.410
Number of reflections	56316
<I/σ(I)>	26	3.9
Completeness [%]	100.0	100
Redundancy	7.3	7.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP			VIA HANGING DROP VAPOR DIFFUSION METHOD AT A RATIO OF 2:1 OF 8.5 MG/ML OF AZYMETRIC VARIANT ABOVE A MOTHER LIQUOR SOLUTION COMPOSED OF 5% (V/V) ETHYLENE GLYCOL, 18% (W/V) POLYETHYLENE GLYCOL 3350, AND 0.15 M AMMONIUM IODIDE WITH AID OF MICROSEEDING.