4BOF
Crystal structure of arginine deiminase from group A streptococcus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
Synchrotron site | Australian Synchrotron |
Beamline | MX1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-12-14 |
Detector | ADSC QUANTUM 210r |
Spacegroup name | P 1 |
Unit cell lengths | 87.910, 92.740, 120.970 |
Unit cell angles | 96.15, 90.27, 100.13 |
Refinement procedure
Resolution | 36.310 - 2.480 |
R-factor | 0.2128 |
Rwork | 0.211 |
R-free | 0.24410 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1s9r |
RMSD bond length | 0.010 |
RMSD bond angle | 1.120 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.850 | 2.570 |
High resolution limit [Å] | 2.480 | 2.480 |
Rmerge | 0.150 | 0.980 |
Number of reflections | 131658 | |
<I/σ(I)> | 10.25 | 1.21 |
Completeness [%] | 99.3 | 94.1 |
Redundancy | 7.4 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5.5 | 0.1M BIS-TRIS PH 5.5, 20% PEG3350, 0.3M LISO4, 25MG/ML PROTEIN |