4BK5
crystal structure of the human EphA4 ectodomain in complex with human ephrin A5 (amine-methylated sample)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 256.950, 256.950, 252.510 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 70.410 - 4.000 |
| R-factor | 0.313 |
| Rwork | 0.313 |
| R-free | 0.32130 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 4BK4 2x11 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.870 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.000 | 4.050 |
| High resolution limit [Å] | 4.000 | 4.000 |
| Rmerge | 0.410 | |
| Number of reflections | 27221 | |
| <I/σ(I)> | 7.7 | 0.59 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 11.3 | 11.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 % W-V POLYETHYLENE GLYCOL 8000 AND 0.1 M TRIS-HCL PH 8.5 | |||
| 1 | 8 % W-V POLYETHYLENE GLYCOL 8000 AND 0.1 M TRIS-HCL PH 8.5 | |||
| 1 | 8 % W-V POLYETHYLENE GLYCOL 8000 AND 0.1 M TRIS-HCL PH 8.5 |
