4BGD
Crystal structure of Brr2 in complex with the Jab1/MPN domain of Prp8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 77 |
| Detector technology | PIXEL |
| Collection date | 2012-05-17 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 107.640, 178.640, 180.400 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 92.370 - 3.100 |
| R-factor | 0.21664 |
| Rwork | 0.214 |
| R-free | 0.26993 |
| Structure solution method | SIRAS |
| Starting model (for MR) | PDB ENTRIES 3HIB AND 2OG4 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.477 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 92.200 | 3.180 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.080 | 0.880 |
| Number of reflections | 63788 | |
| <I/σ(I)> | 9.8 | 1.4 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 4.2 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 100 MM HEPESNA PH 7.5, 200 MM MGCL2, 32-40% PEG400 |
