4BEA
Crystal Structure of eIF4E in Complex with a Stapled Peptide Derivative
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER X8 PROTEUM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-10-21 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 38.301, 91.677, 137.908 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.880 - 2.570 |
| R-factor | 0.22232 |
| Rwork | 0.220 |
| R-free | 0.26575 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4aza |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.355 |
| Data reduction software | PROTEUM2 |
| Data scaling software | PROTEUM2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 68.950 | 2.630 |
| High resolution limit [Å] | 2.570 | 2.570 |
| Rmerge | 0.090 | 0.460 |
| Number of reflections | 8036 | |
| <I/σ(I)> | 11.08 | 2.33 |
| Completeness [%] | 98.8 | 83.2 |
| Redundancy | 10.15 | 2.64 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | 18-26% OF POLYETHYLENE GLYCOL 3350, 0.01-02M AMMONIUM SULPHATE AND 100MM BIS-TRIS AT PH 5.5 |
