4BB2
Crystal structure of cleaved corticosteroid-binding globulin in complex with progesterone
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-02-12 |
Detector | MARRESEARCH |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 89.190, 122.700, 81.930 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.070 - 2.480 |
R-factor | 0.18293 |
Rwork | 0.177 |
R-free | 0.23393 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2vdy |
RMSD bond length | 0.007 |
RMSD bond angle | 1.276 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.000 | 2.630 |
High resolution limit [Å] | 2.480 | 2.480 |
Rmerge | 0.120 | 0.600 |
Number of reflections | 16184 | |
<I/σ(I)> | 12.9 | 3.2 |
Completeness [%] | 99.6 | 97.6 |
Redundancy | 7.2 | 7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | 0.1 M HEPES 7.5, 2 % PEG 400, 2 M AMMONIUM SULFATE |