4BAY
Phosphomimetic mutant of LSD1-8a splicing variant in complex with CoREST
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-1 |
Synchrotron site | ESRF |
Beamline | ID14-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-12-12 |
Detector | ADSC QUANTUM 210 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 117.980, 179.120, 233.640 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 59.060 - 3.100 |
R-factor | 0.20901 |
Rwork | 0.209 |
R-free | 0.22583 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2x0l |
RMSD bond length | 0.005 |
RMSD bond angle | 0.907 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0027) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 65.000 | 3.270 |
High resolution limit [Å] | 3.100 | 3.100 |
Rmerge | 0.100 | 0.600 |
Number of reflections | 44180 | |
<I/σ(I)> | 11.8 | 2.6 |
Completeness [%] | 99.7 | 99.7 |
Redundancy | 4.5 | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 20DEG BY HANGING-DROP VAPOR DIFFUSION METHOD BY MIXING EQUAL VOLUMES OF PROTEIN SAMPLES WITH RESERVOIR SOLUTIONS CONTAINING 1.2 M SODIUM/POTASSIUM TARTRATE AND 100 MM N-(2-ACETAMIDO)IMINODIACETIC ACID, PH 6.5 |