4B8Z
Crystal structure of human GDP-L-fucose synthase with bound NADP and GDP, rhombohedral crystal form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-12-15 |
| Detector | MARRESEARCH |
| Spacegroup name | H 3 |
| Unit cell lengths | 91.447, 91.447, 429.130 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 77.880 - 2.750 |
| R-factor | 0.2347 |
| Rwork | 0.233 |
| R-free | 0.26100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gfs |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.910 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 77.880 | 2.900 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Rmerge | 0.060 | 0.530 |
| Number of reflections | 34584 | |
| <I/σ(I)> | 13.8 | 2 |
| Completeness [%] | 99.3 | 100 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.3M NABR, 0.1M BIS-TRIS-PROPANE (PH 7.5), 23% PEG3350, 10% ETHYLENE GLYCOL |
