4B8W
Crystal structure of human GDP-L-fucose synthase with bound NADP and GDP, tetragonal crystal form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2009-12-12 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 42 |
| Unit cell lengths | 111.470, 111.470, 46.920 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.320 - 2.750 |
| R-factor | 0.2035 |
| Rwork | 0.201 |
| R-free | 0.25240 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gfs |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.920 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.320 | 2.900 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Rmerge | 0.110 | 0.680 |
| Number of reflections | 14273 | |
| <I/σ(I)> | 9.2 | 1.3 |
| Completeness [%] | 93.5 | 93.7 |
| Redundancy | 3.1 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.2 M NACL, 0.1 M BIS-TRIS PH 5.5, 25% PEG 3350 |
