4B7L

Crystal Structure of Human Filamin B Actin Binding Domain with 1st Filamin Repeat

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	120
Detector technology	IMAGE PLATE
Collection date	2011-01-14
Detector	RIGAKU RAXIS IV
Spacegroup name	P 1 21 1
Unit cell lengths	72.180, 69.690, 73.460
Unit cell angles	90.00, 111.10, 90.00

Refinement procedure

Resolution	35.738 - 2.050
R-factor	0.185
Rwork	0.183
R-free	0.21900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2wa5
RMSD bond length	0.002
RMSD bond angle	0.598
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.700	2.160
High resolution limit [Å]	2.050	2.050
Rmerge	0.110	0.560
Number of reflections	41595
<I/σ(I)>	8	2.5
Completeness [%]	97.5	95.6
Redundancy	4.2	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		8.5		30% PEG 4000, 0.1 M TRIS HCL PH 8.5, 0.2 M SODIUM ACETATE