4B52
Crystal structure of Gentlyase, the neutral metalloprotease of Paenibacillus polymyxa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 120 |
| Detector technology | PIXEL |
| Collection date | 2011-09-05 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.121, 77.021, 64.443 |
| Unit cell angles | 90.00, 103.58, 90.00 |
Refinement procedure
| Resolution | 48.600 - 1.760 |
| R-factor | 0.19768 |
| Rwork | 0.195 |
| R-free | 0.24749 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3fvp |
| RMSD bond length | 0.024 |
| RMSD bond angle | 2.136 |
| Data reduction software | XDS |
| Data scaling software | SADABS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0119) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.600 | 1.860 |
| High resolution limit [Å] | 1.760 | 1.760 |
| Rmerge | 0.110 | 0.670 |
| Number of reflections | 57590 | |
| <I/σ(I)> | 7.9 | 1.6 |
| Completeness [%] | 97.5 | 97.5 |
| Redundancy | 3.3 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.2M NACL, 0.1 M HEPES PH 7.5, 25% PEG 3350 |
