4B4O
Crystal Structure of human epimerase family protein SDR39U1 (isoform2) with NADPH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-04-25 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 |
| Unit cell lengths | 80.824, 83.362, 105.191 |
| Unit cell angles | 89.93, 76.73, 71.52 |
Refinement procedure
| Resolution | 102.070 - 2.700 |
| R-factor | 0.19958 |
| Rwork | 0.198 |
| R-free | 0.23074 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.539 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.850 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.180 | 0.570 |
| Number of reflections | 68174 | |
| <I/σ(I)> | 11.9 | 4.1 |
| Completeness [%] | 98.2 | 93.8 |
| Redundancy | 7.9 | 7.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.2M NA FORMATE 15W/V PEG 3350 |
