4B36
Crystal Structure of Human Angiogenin with an Engineered Loop Exhibits Conformational Flexibility at the Functional Regions of the Molecule
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX10.1 |
Synchrotron site | SRS |
Beamline | PX10.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-11-04 |
Detector | MARMOSAIC 225 mm CCD |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 38.353, 54.676, 94.731 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.151 - 1.760 |
R-factor | 0.2389 |
Rwork | 0.236 |
R-free | 0.28530 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ang |
RMSD bond length | 0.007 |
RMSD bond angle | 1.012 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.820 |
High resolution limit [Å] | 1.760 | 1.760 |
Rmerge | 0.080 | 0.300 |
Number of reflections | 20481 | |
<I/σ(I)> | 14.5 | 2.9 |
Completeness [%] | 91.9 | 65.8 |
Redundancy | 4.8 | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 1.75-2.5 M NACL |