4AZI
Differential inhibition of the tandem GH20 catalytic modules in the pneumococcal exo-beta-D-N-acetylglucosaminidase, StrH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARMOSAIC 325 mm CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 67.060, 116.040, 69.640 |
| Unit cell angles | 90.00, 109.57, 90.00 |
Refinement procedure
| Resolution | 43.470 - 1.980 |
| R-factor | 0.24398 |
| Rwork | 0.241 |
| R-free | 0.30201 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2yl5 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.168 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.100 | 2.090 |
| High resolution limit [Å] | 1.980 | 1.980 |
| Rmerge | 0.130 | 0.360 |
| Number of reflections | 68111 | |
| <I/σ(I)> | 6.4 | 3.2 |
| Completeness [%] | 97.8 | 97.1 |
| Redundancy | 4.3 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
