4AZG
Differential inhibition of the tandem GH20 catalytic modules in the pneumococcal exo-beta-D-N-acetylglucosaminidase, StrH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARMOSAIC 325 mm CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 66.700, 115.200, 129.900 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.400 |
| R-factor | 0.19334 |
| Rwork | 0.190 |
| R-free | 0.24751 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2yl5 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.369 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.530 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.120 | 0.400 |
| Number of reflections | 39589 | |
| <I/σ(I)> | 15.4 | 5.4 |
| Completeness [%] | 99.1 | 97.3 |
| Redundancy | 10 | 9.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
