4AZF
Human DYRK2 in complex with Leucettine L41
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-09-07 |
| Detector | ADSC CCD |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 83.461, 83.461, 148.534 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 74.270 - 2.550 |
| R-factor | 0.19973 |
| Rwork | 0.197 |
| R-free | 0.25515 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3k2l |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.378 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 59.020 | 2.690 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Rmerge | 0.120 | 0.880 |
| Number of reflections | 17843 | |
| <I/σ(I)> | 12 | 2.5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.9 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.2 M NA/KPO4, 20% PEG 3350, 10% ETHYLENE GLYCOL, pH 7.5 |
