4AZB
Differential inhibition of the tandem GH20 catalytic modules in the pneumococcal exo-beta-D-N-acetylglucosaminidase, StrH
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | MARMOSAIC 325 mm CCD |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 90.070, 90.070, 101.600 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 41.170 - 2.100 |
R-factor | 0.17494 |
Rwork | 0.173 |
R-free | 0.20824 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2yl6 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.109 |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.030 | 2.210 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.090 | 0.390 |
Number of reflections | 28340 | |
<I/σ(I)> | 18.8 | 6.5 |
Completeness [%] | 100.0 | 100 |
Redundancy | 10.7 | 10.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |