4AVX
Hepatocyte Growth Factor-Regulated Tyrosine Kinase Substrate (Hgs-Hrs) bound to an IP2 compound at 1.68 A Resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-11-02 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 27.825, 67.545, 57.423 |
| Unit cell angles | 90.00, 103.51, 90.00 |
Refinement procedure
| Resolution | 55.830 - 1.680 |
| R-factor | 0.18357 |
| Rwork | 0.181 |
| R-free | 0.23103 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3zyq |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.703 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.030 | 1.710 |
| High resolution limit [Å] | 1.680 | 1.680 |
| Rmerge | 0.040 | 0.180 |
| Number of reflections | 22910 | |
| <I/σ(I)> | 11.6 | 3.7 |
| Completeness [%] | 97.0 | 93.3 |
| Redundancy | 2.3 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1 M BIS-TRIS PH 5.5; 25% PEG 3350 |
