4AU7
The structure of the Suv4-20h2 ternary complex with histone H4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2012-03-10 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 37.270, 65.170, 209.490 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 55.330 - 2.070 |
| R-factor | 0.19498 |
| Rwork | 0.193 |
| R-free | 0.24039 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3rq4 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.875 |
| Data reduction software | XDS |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.300 | 2.130 |
| High resolution limit [Å] | 2.100 | 2.070 |
| Rmerge | 0.040 | 0.360 |
| Number of reflections | 29593 | |
| <I/σ(I)> | 16.9 | 2 |
| Completeness [%] | 97.3 | 82 |
| Redundancy | 3.4 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | PROTEIN WAS CRYSTALLIZED FROM 9% PEG 3350, 0.1 M HEPES PH 6.5 |
