4ASF
The structure of modified benzoquinone ansamycins bound to yeast N- terminal Hsp90
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-12-06 |
| Detector | RIGAKU CCD |
| Spacegroup name | P 43 2 2 |
| Unit cell lengths | 73.734, 73.734, 109.363 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 61.210 - 2.600 |
| R-factor | 0.20418 |
| Rwork | 0.200 |
| R-free | 0.29225 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | IN-HOUSE MODEL |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.899 |
| Data reduction software | CrystalClear |
| Data scaling software | CrystalClear |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.140 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.080 | 0.530 |
| Number of reflections | 9800 | |
| <I/σ(I)> | 12.4 | 2.6 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 8.02 | 7.45 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | pH 7 |
