4ARC
Ternary complex of E. coli leucyl-tRNA synthetase, tRNA(leu) and leucine in the editing conformation
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-06-15 |
Detector | ADSC QUANTUM 315r |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 77.080, 119.370, 141.100 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.570 - 2.000 |
R-factor | 0.21229 |
Rwork | 0.210 |
R-free | 0.25720 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1h3n |
RMSD bond length | 0.014 |
RMSD bond angle | 1.678 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.6.0116) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.100 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.070 | 0.720 |
Number of reflections | 87917 | |
<I/σ(I)> | 10.5 | 2 |
Completeness [%] | 99.3 | 99.1 |
Redundancy | 3.9 | 3.76 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5.6 | THE TERNARY COMPLEX ECLEURS-TRNA-LEU WAS CRYSTALLIZED FROM 0.1 M SODIUM ACETATE (PH 5.6), 14-18% (W/V) PEG 6000 AND 200 MM NACL. THE CRYSTALS WERE FROZEN IN LIQUID NITROGEN USING 22% (V/V) ETHYLENE GLYCOL AS CRYOPROTECTANT |