4AQE
CRYSTAL STRUCTURE OF DEAFNESS ASSOCIATED MUTANT MOUSE CADHERIN-23 EC1- 2S70P AND PROTOCADHERIN-15 EC1-2 FORM I
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-06-29 |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 174.023, 40.866, 84.775 |
| Unit cell angles | 90.00, 102.95, 90.00 |
Refinement procedure
| Resolution | 22.690 - 2.270 |
| R-factor | 0.17601 |
| Rwork | 0.173 |
| R-free | 0.23873 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4apx |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.404 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.690 | 2.330 |
| High resolution limit [Å] | 2.270 | 2.270 |
| Rmerge | 0.130 | 0.440 |
| Number of reflections | 27207 | |
| <I/σ(I)> | 9.6 | 2.3 |
| Completeness [%] | 99.2 | 96.8 |
| Redundancy | 3.5 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 0.1 M MES, 12% PEG 4000, PH 6.5 |
