4AP2
Crystal structure of the human KLHL11-Cul3 complex at 2.8A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-12-14 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 147.536, 40.162, 234.758 |
| Unit cell angles | 90.00, 107.36, 90.00 |
Refinement procedure
| Resolution | 46.940 - 2.800 |
| R-factor | 0.2059 |
| Rwork | 0.204 |
| R-free | 0.23560 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.890 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.970 | 2.950 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.110 | 1.120 |
| Number of reflections | 33319 | |
| <I/σ(I)> | 12.2 | 2.2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 9 | 9.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 25% PEG3350, 0.15M NAI, 8% ETHGLY |
