4AOT
Crystal Structure of Human Serine Threonine Kinase-10 (LOK) Bound to GW830263A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-02-18 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 55.690, 87.510, 148.590 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 75.400 - 2.330 |
| R-factor | 0.21877 |
| Rwork | 0.217 |
| R-free | 0.25221 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2j7t |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.593 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.640 | 2.420 |
| High resolution limit [Å] | 2.330 | 2.330 |
| Rmerge | 0.135 | 1.190 |
| Number of reflections | 31773 | |
| <I/σ(I)> | 9.8 | 2.1 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 6.1 | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 0.2M NAI, 0.1M BISTRISPROPANE PH 6.5, 20% PEG3350, 10% ETHYLENE GLYCOL |
